News:

  • 2024/04/16:

    Inspiring and long-lasting collaboration with Daniel Khananshvili and Moshe Giladi from the Tel Aviv University on Na+/Ca2+ exchangers resulted in a nice joint paper in Commun Biol. Thank you guys for your hard work and persistence!

  • 2024/04/15:

    The 15th EFTMS workshop will start soon - on Wed, Apr 17, 2024. Dont miss the lecture of prof. Michael L. Gross lecture available also online. Check here.

  • 2024/03/21:

    Further insight into the function of an AsLOV2-FMN based singlet oxygen production provieded through a great collaboration with Erik Sedlak. Now published in Protein Science

  • 2024/03/08:

    Fantastic news - our collaboration with the Haitin group (Yoni and Moshe) brought surprising insights into the function of enigmatic intracellular chloride channels. And the sweet reward - the story made it to Nat Commun!

  • 2024/03/06:

    We have two more PhD position available. One on structural mass spec of prion proteins and membrane transporters and the other on clinical mass scpetrometry and method development. Apply till Apr 20!

  • 2024/03/04:

    Big welcome to Petr Jerabek. He has joined our group as a programmer and will help us with the development of fancy tools for MS data analysis in collaboration with Bruker.

  • 2024/02/14:

    We have a new visitor in our lab - Francesca Del Cioppo from the Uni Roma.

  • 2024/01/26:

    🕷🕷🕷Our lab ZOO is growing again. Mike's successor has just arrived! Welcome ❤ Mike II.

  • 2024/01/20:

    In 2023 we spent a lot of time working on different projects with Angel L. Pey from Uni of Grenada. Now the results are coming out. First one on AGT1 mutants is published in FEBS Lett. Thanks INSTRUCT and CIISB for support.

  • 2024/01/18:

    We are looking for PhD students! Position funded for 4 years in the field of structural MS and cool FT-MS top-down analysis - details here.

Horizon 2020
EPIC-XS
EU FT-ICR MS MSCA

Important:

News

2024/04/16:

Inspiring and long-lasting collaboration with Daniel Khananshvili and Moshe Giladi from the Tel Aviv University on Na+/Ca2+ exchangers resulted in a nice joint paper in Commun Biol. Thank you guys for your hard work and persistence!

2024/04/15:

The 15th EFTMS workshop will start soon - on Wed, Apr 17, 2024. Dont miss the lecture of prof. Michael L. Gross lecture available also online. Check here.

2024/03/21:

Further insight into the function of an AsLOV2-FMN based singlet oxygen production provieded through a great collaboration with Erik Sedlak. Now published in Protein Science

2024/03/08:

Fantastic news - our collaboration with the Haitin group (Yoni and Moshe) brought surprising insights into the function of enigmatic intracellular chloride channels. And the sweet reward - the story made it to Nat Commun!

2024/03/06:

We have two more PhD position available. One on structural mass spec of prion proteins and membrane transporters and the other on clinical mass scpetrometry and method development. Apply till Apr 20!

2024/03/04:

Big welcome to Petr Jerabek. He has joined our group as a programmer and will help us with the development of fancy tools for MS data analysis in collaboration with Bruker.

2024/02/14:

We have a new visitor in our lab - Francesca Del Cioppo from the Uni Roma.

2024/01/26:

🕷🕷🕷Our lab ZOO is growing again. Mike's successor has just arrived! Welcome ❤ Mike II.

2024/01/20:

In 2023 we spent a lot of time working on different projects with Angel L. Pey from Uni of Grenada. Now the results are coming out. First one on AGT1 mutants is published in FEBS Lett. Thanks INSTRUCT and CIISB for support.

2024/01/18:

We are looking for PhD students! Position funded for 4 years in the field of structural MS and cool FT-MS top-down analysis - details here.

2024/01/17:

The last piece of Marok's PhD thesis puzzle is now in Anal. Chem. Well done Marok!

2024/01/13:

Welcome Honza and enjoy fun with structural mass spec!

2024/01/13:

Congratulations to Marek on his first first-author paper. Deep insight into FPOP data processing in now in J. Proteome. Res. .

2024/01/10:

We have two ERASMUS visitors from the University of Lisbon who will work with us on FT-ICR MS. Welcome Marta and Inês!

2024/01/03:

Our group is growing. Welcome Ženya!

2023/12/13:

We are organizing 15th EU FTMS workshop! It will take place in Prague on Apr 17-20, 2024. Check the web site for more information.

2023/11/03:

Great news, Ljubi is back after maternity leave 👶 👶 !

2023/10/26:

Alan's contribution to the desing of sci-fi SPIDOC instrumentation - single particle imaging of protein complexes. Out in the Journal of Instrumentation. Cool!

2023/10/26:

Fruits of Maartje's work in our lab is starting to pop-out. Top-down 2D MS for proteoform characterization, now in Anal Chem.

2023/10/02:

If you want something really cool, let THE BOSS do the work! Great interlaboratory work on the online deglycosylation in HDX and proteomics now out in JASMS.

2023/09/14:

Great news! Our JACS paper on FFAP technology was awarded Hanus Prize 2023 Congratulations to Kuky, Luky and all others.

2023/09/07:

New MMB chapter on our HDX workflow full of tips and tricks, especially on immobilized protease columns. This time applied to antibodies and from Zuza.

2023/08/25:

Another masterpiece from the ongoing collaboration with the Veverka group!

2023/07/18:

Good bye Maartje, thank you for teaching us 2DMS and good luck in Rouen!

2023/06/29:

Zuza's lucky week - state doctoral exam done and second paper out!

2023/06/01:

Congratulations to Valča on a successful MSc defense and good luck in future PhD!

2023/05/15:

Congratulations to Terka on the best IMIC master thesis in 2022!

2023/05/15:

MALDI chips rocks! Pepa's first paper is out in Clinical Proteomics!

2023/04/13:

Thrilling collaboration with Klara Hlouchova on “non-existing proteins”. Our achievements got well accepted in Nat Ecol Evol. Thanks EPIC-XS for support.

2023/04/12:

And yet another one piece with Angel Pey and Milagros Medina. Our collaborative effort on NQO1 active site mutations is now fully published in FEBS J. And again thanks EU FT-ICR MS (H2020) for support.

2022/10/13:

This year it looks like a solid harvest of work conducted with Angel L. Pey from Uni of Grenada. Another joint paper, this time on structural effects induced by cavity making mutation on a two domain protein - PGK1. Paper now available in Sci Rep. Thanks EU FT-ICR MS (H2020) for support.

2022/10/10:

Congratulations to Lukas on BIOCEV's PhD Student Publication Award - our JACS paper on FFAP attracts attention 😉. Great collaboration with the Beier group and CF Plus Chemicals. Thanks CSF and H2020 for financial support and HPST for sponsoring this award.

2022/09/13:

Insight into the role of site-specific phosphorylation of human antioxidant and disease-associated flavoprotein NQO1 provided through great collaboration with teams of Angel L. Pey and Milagros Medina. Paper now available in Arch Biochem Biophys. Thanks EU FT-ICR MS (H2020) for support.

2022/09/09:

Congratulations to Bára on succesfull (and very nice) master thesis defense!

2022/08/30:

Nice work with Moshe and Yoni, this time on tweety proteins is finally out. Freely available in Commun. Biol.! Emelia and guys, thanks for great collaboration and thanks EU FT-ICR MS (H2020) for support.

2022/07/21:

Our work on top-down FPOP, highlighting the benefits of exact gas-phase isolation of selected protein species and multiple fragmentation modes, is now available in the Analytical Chemistry!

2022/06/22:

Another popularization event with Aleš & Co. participation - Věda Fest!

2022/06/10:

Congratulations to Jasmína on Bc thesis defense! Happy she stays with us and will work on HDX of membrane proteins.

2022/06/10:

Congratulations to Aleš on becoming a group leader at the 1st Faculty of Medicine where he will continue with Víťa in their adventures. Good luck! We enjoyed the time together and are glad that we stay in close contact and will still work together.

2022/06/04:

Our PhDs lead by Aleš were presenting at the Science Fair (Veletrh vědy). Great event! Some pictures here - one, two, three... ;)

2022/06/02:

Another great paper on NQO1, now about cavity making mutantions and allostery in flavoproteins. Excellent multilaboratory collaboration lead by Angel L. Pey from the University of Granada. Thanks to EPIC-XS H2020 Project for support!

2022/05/31:

Congratulations to Terka on very nice master thesis defense! We wish her good luck on her PhD in the Heck's lab! 😲

2022/05/19:

This is big. Fantastic collaboration with Moshe and Yoni on cis-prenyltransferase continues with another great outcome. Paper now published in Sci. Adv. reveals how these enzymes synthesize long products via small active site!

2022/02/14:

Happy to help Klara and her team (KH lab) with the work deciphering evolution of early protein-RNA interaction. Paper supported by Terka and The Boss is now in high-ranking Mol. Biol. Evol. journal!

2022/02/09:

Marok's work on FPOP combined with top-down for transcription factor::dsDNA interaction is now published in Anal. Chem.! Very nice complement to our previus HDX and X-linking studies, now with residue level resolution.

2021/12/08:

Our team is growing! Welcome Maartje and Michael! We now have two real experts to expand MS into a second dimension and shoot the protein ions with all electron and laser guns we have. Top-down era of our lab is now making a huge leap forward.

2021/12/06:

Happy news! We received funding from the Czech Science Foundation to expand structural proteomics tools by developing new reagents, enhancing the existing ones and diving into transmembrane proteins.

2021/12/01:

Great success of Jarin and Fiji who have their protein fast-fluoralkylation tricks now published in prestigious  J Am Chem Soc. Also thanks for fruitful collaboration - Beier group and CF Plus Chemicals.

2021/11/25:

Highly productive collaboration with Angel Pey led to another publication on mammalian NQO1 flavoproteins. This time on phosphorylation and a specific evolutionary divergent mutation close to it. Available in FEBS Letters. Thanks to EU_FT-ICR_MS H2020 Project for support!

2021/11/04:

Our systematic structural characterization of haptoglobin-hemoglobin interaction resulted in a first paper, Hp-Hb interaction studied by FPOP.

2021/09/23:

Congratulations to Honza (Fiji), now officially PhD in biochemistry!

2021/09/22:

Active and productive collaboration with Sergei Strelkov's lab resulted in another paper on lamin assembly. Work was done under EU_FT-ICR_MS H2020 Project.

2021/09/01:

After several years of post-docking in Hamburg, Alan returns to our lab. Welcome home Alan!

2021/08/20:

Great collaboration with Angel Luis Pey from the University of Granada resulted in a very nice multidisciplinary study on the effect of mutations in human flavoproteins. Now available in Redox Biology! Thanks to EU_FT-ICR_MS H2020 Project for support!

2021/06/15:

We are organizing 2nd Advanced User School under the H2020 EU_FT-ICR_MS consortium. The school will be held in Prague/Vestec, Czech Republic on September 26-30, 2021 and will be stuffed with basics and applications of FT-ICR MS. Participation fee, accommodation and meals covered for 50 selected participants. Registration form can be found HERE. Deadline for application - Aug 23, 2021. Entry to CZ is regulated according to actual COVID situation.

2021/05/31:

Petr's collaboration with the Volf's lab and Austrian's parasitologists is now published in Parasit. Vectors.

2021/04/21:

Proteomic analysis of F. pinicola secretome leading to expression and characterization of two laccases (collaboration with the Ludwig Lab) has just been accepted in Enzyme Microb Technol

2021/04/09:

Structural analysis of human haptoglobin by FPOP and the benefits of ion mobility (TIMS/PASEF) for such analysis is now published in ACS Omega

2021/03/25:

Structural aspects of Von Willebrand factor binding and processing by metallopeptidase ADAMTS-13 studiedby integrative structural biology. Trans-national access via the EPIC-XS resulted in a joint publication in J. Mol. Biol.

2021/03/10:

The results of collaboration with the parasitology groups from Charles University and Morroco are now published in Transboundary and Emerging Diseases

2021/03/08:

Our software LinX for interpretation of chemical cross-linking data, with special features for homodimer elucidation or protein:DNA cross-links is now available on GitHub and related paper published in J Prot Res

2021/02/05:

Nice colaboration with Erik Sedlak's group on cytochrome c oxidation is now available in Int J Biol Macromol. Thanks EU_FT-ICR_MS H2020 Project for supporting this trans-national access! Enjoy 50 days of free access using the link above (till Mar 27, 2021).

2021/01/31:

New joint paper with Ales, from the times he was not part of our lab. FEBS J paper on Ku70/80 intramolecular interactions combining cryoEM and structural MS.

2021/01/08:

Collaboration with the Ludwig lab resulted in another paper on wood degrading enzymes. This time about chimeric CDHs in ACS Catalysis.

2020/12/18:

Paper on TEAD1-DNA interaction is now published in Structure. Enjoy 50 days of free access (till Feb 4, 2021).

2020/12/14:

Congratulations to Paja, now officially PhD in biochemistry!

2020/12/11:

Our HDX workflow combined with Ruza's experience on protein-DNA interaction studies was compiled into Methods Mol. Biol. chapter that has just been published.

2020/11/20:

Two new papers employing MALDI biotyping of sand flies are now in Parasit. Vectors. Collaboration of Petr with the Volf's lab.

2020/10/19:

Pleasant colaboration with Haitin Lab on cis-prenyltrasferase made it from BioRXiv to Nature Communications!. Thanks EU_FT-ICR_MS H2020 Project for support!

2020/10/12:

Petr contributed to another community-wide paper stuffed with ideas and recommendations toward transparency and accessibility of data from cross-linking mass spectrometry. Check the full text!

2020/09/24:

Congratulations to Franta, now officially PhD in biochemistry!

2020/09/17:

Collaborative work with the team of Vaclav Veverka is now available in Structure.

2020/09/09:

Karel Vališ joined the Reviewer Board of Metabolites.

2020/09/07:

3D printing of MALDI targets is now published in Analytical Chemistry.

2020/09/07:

Files for hands-on sessions at 21. Czech MS School are available under Downloads.

2020/07/14:

Collaboration with Sergei Strelkov's lab on lamin assembly is now published. Work was done under EU_FT-ICR_MS H2020 Project.

2020/06/28:

We are organizing 7th Short Course Structural Proteomics: Analysis of protein surface accessibility by Top Down mass spectrometry under the H2020 EU_FT-ICR_MS consortium. The course will be held in Prague/Vestec, Czech Republic on August 16-19, 2020. There are still free places so do not hesitate to apply. Please be aware that travel restriction may apply and the situation may change till half of August.

2020/06/18:

Nice work and pleasant collaboration with the Haitin Lab on soluble chloride channel Clic-5 now out in The FASEB J.
Thanks EU_FT-ICR_MS H2020 Project for support!

2020/06/12:

Another collaborative work with Haitin Lab done under EU_FT-ICR_MS H2020 Project this time on cis-prenyltrasferase is available as preprint on bioRXiv.

2020/06/01:

Ales Hnizda joined our lab. He is funded by Marie Sklodowska-Curie Action Individual Fellowship (Widening). Welcome Ales!

2020/05/05:

Collaboration with RECAMO team on heat shock proteins and co-chaperones resulted in another paper The interaction of the mitochondrial protein importer TOMM34 with HSP70 is regulated by TOMM34 phosphorylation and binding to 14-3-3 adaptors. which is now available in J Biol Chem.

2020/05/03:

New review Targeting ERK-Hippo Interplay in Cancer Therapy. from Karel is now published in Int. J. Mol. Sci..